Chicken industry has important role in food supply as one of protein source in Indonesia. West Java province has largest population of chicken poultry, with Bogor, Sukabumi, and Cianjur have the highest chicken producer. In order to maintain the production, farmers used antibiotics for prophylaxis, growth promotor, and therapy. However, extensive use of antibiotic can increase thenumber of antibiotic resistant bacteria. Staphylococcus aureus, Streptococcus spp, and Klebsiella spp are the example of flora normal in chicken that affected with abusive use of antibiotic. The aim of this study was to determine the antibiotic resistance profile of S. aureus, Streptococcus spp., and Klebsiella spp. isolated from cloacal swab of chicken poultry in Bogor, Sukabumi, and Cianjur, West Java Indonesia. The result of antibiotics susceptibility test showed that S. aureus was resistant to ampicillin (98%), erythromycin (95%), nalidixic acid (93%), tetracycline (92%), oxytetracycline (90%), enrofloxacin (69%) ciprofloxacin (56%) and still sensitive to gentamycin and chloramphenicol. Streptococcus spp. was resistant to tetracycline (100%), doxycycline (87.5%), and sensitive to chloramphenicol and ampicillin. Klebsiella spp. was resistant to erythromycin (100%), ampicillin (94.83%) oxytetracycline (93.10%), tetracycline and nalidixic acid (89.66%), enrofloxacin (86.21%), ciprofloxacin (81.03%), 55.17% to gentamycin and 29.31% to chloramphenicol.