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Extensive use of antibiotics on farms play a major role in the development of commensal and pathogenic bacterial resistance. The resistance characteristic of bacteria is brought by genes which can spread cell-to-cell. Escherichia coli is normal bacteria in poultry gut. The purpose of this study was to isolate and identify E. coli, test E. coli against antibiotics and detect genes encoding antibiotics resistance. A total of 30 cloacal swab samples were collected from layer farm in Bogor Districts. Seventeen E. coli isolates were identified. Antibiotics susceptibility testing used disk diffusion Kirby-Bauer method. Tetracycline, oxytetracycline, ampicillin, gentamycin, nalidixic acid, ciprofloxacin, enrofloxacin, chloramphenicol and erythromycin were used in this study. Antibiotics resistance were tetracycline (94.1%), oxytetracycline (94.1%), ampicillin (100%), gentamycin (70.6%), nalidixic acid (88.2%), ciprofloxacin (41.2%), enrofloxacin (41.2%), chloramphenicol (0%) and erythromycin (100%). The distribution of antibiotics resistance genes were tetA (81.3%), blaTEM (100%), aac(3)-IV (8.3%), gyrA (100%) and ermB (0%). In conclusion, E. coli had been isolated from broiler poultry farm in Bogor Districts. It was resistant to tetracycline, oxytetracycline, ampicillin, gentamycin, nalidixic acid, ciprofloxacin, enrofloxacin and erythromycin. The tetA, blaTEM, aac(3)-IV and gyrA genes were detected in E. coli isolates.