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Diazinon is an organophosphate class pesticide which is widely used in agricultural business. Diazinone has cytotoxic and pathological effects that work in the body with the effect of inhibiting the acetylcholinesterase enzyme, causing acetylcholine buildup in the synapse gap which can lead to cell apoptosis and hormonal disturbances in the reproductive system. The purpose of this study was to determine the effectiveness of black cumin seeds as an antioxidant to reduce the negative impact of free radicals in the body and to suppress caspase-3 expression as a caspase mediator for apoptosis in the sertoli cells as a producer of substances needed in the spermatogenesis process in the histopathological picture of the testes. This study was using male Balb / c mice which were divided into 5 treatment groups, namely negatif control (K-) for untreated male mice; positive control (K+) for male mice given diazinone 40 mg / kg BW for 8 weeks; whereas treatments 1, 2, and 3 (P1, P2, and P3) were given exposure to diazinon 40 mg / kg BW for 8 weeks and black cumin extract 200, 400, and 600 mg / kg BW at week 5-8, respectively.  The results showed that giving black cumin seed extract for 3 weeks had an effect on decreasing caspase-3 expression and increasing the number of sertoli cells.

Black Cumin Seeds, Diazinon, Sertoli Cells, Caspase-3