Lead as one of the most common and hazardous heavy metals for humans and animals. The purpose of this research was to determine the effect of white guava (Psidium guajava) fruit juice on the seminiferous tubule diameter and epithelium thickness in rats (Rattus norvegicus). The research design was completely randomized design (CRD). The number of samples that was used are 25 male rats with average weight 200 grams. There are 5 groups of treatment consisted of 5 rats each group including: the control group (C) was administered with distilled water orally; the T0 group was induced with lead acetate (50 mg/Kg BW) orally; the T1, T2, T3 group were induced with lead acetate (50 mg/Kg BW) and after 4 hours, it was administered with 25%, 50%, 100% concentration of white guava fruit juice, respectively. All the treatments were conducted for 14 days. The histopathology slides of testis were made with HE staining and continued with the measurement of seminiferous tubules diameter and epithelium thickness. The data were analyzed using One Way ANOVA and Duncan test (p<0.05). The research result showed that control group (C) which was given distilled water only showed 336.24±23.32 µm and 66.46±4.39 µm as the diameter and epithelium thickness od seminiferous tubules and  the  T0 group which induced by lead acetate only decreased the value of diameter and epithelium thickness of seminiferous tubules (243.38±49.35 µm and 44.08±14.45 µm). The T1, T2 and T3 groups showed positive effects on the diameter (323.49±22.82 µm; 314.41±13.04 µm; 325.04±16.88 µm, respectively) and epithelium thickness (56.36±3.36 µm; 60.50±3.81 µm; 66.744±9.50 µm, respectively). There was no significant difference between each group. The administration of guava juice to rats induced with lead acetate can show a positive effect to diameter and epithelium thickness of seminiferous tubules.