Colibacillosis is one of the most important infection in Indonesia’s poultry industry. Colibacillosis is caused with avian pathogenic Escherichia coli (APEC), and causes airsaculitis, perihepatitis, and pericarditis. APEC serotypes which the most infected in poultry are O1K1, O2K1, and O78K80. Antibiotic have been widely used to eliminate E. coli infetion in poultry farm in recent years. However, high frequency of treatment with antibiotics was cause resistance. The aims of this study were to find out sensitivity of 5 APEC isolates to antibiotics. Five APEC isolates, serotypes O1K1, O2K1, O78K80, O157H7, and unknown serotype were used to antibiotics sensitivity examination (amikacin, kanamycin, ampicillin, trimethoprim, and chlorampenicol) in Mueller Hinton Agar (MHA). Sensitivity profiles against 5 different antibiotics of 5 APEC isolates were determined by using Kirby-Bauer method according to Clinical Laboratory Standard Institute (CLSI). The conclusion, antibiotics sensitivity of amikacin, kanamycin, ampicillin, trimethoprim, and chlorampenicol respectively was 80%, 80%, 0%, 100%, and 100%. Most of antibiotics in this study was used to colibacillosis treatment, except ampicillin.