Acute kidney injury is a loss of kidney function characterized by decreased kidney function, decreased glomerular filtration, and increased levels of urea and creatinine. Blueberry extract has a high anthocyanin content which can act as an antioxidant and anti-inflammatory, making it a potential candidate for natural therapy for acute kidney injury. The purpose of this study was to determine the role of blueberry extract on TNF-α expression and the number of inflammatory cells in acute kidney injury. Rats (Ratus norvegicus) male Wistar stock aged 6-8 weeks, body weight between 200-250 grams, 20 rats were divided into 5 groups consisting of a negative control group, namely mice without treatment, a positive group namely mice induced acute kidney injury by glycerol 50%, and therapy groups P1, P2, P3 induced acute kidney injury and were given blueberry extract at doses of 500 mg/kg, 1000 mg/kg, and 1500 mg/kg. Blueberry extract was given orally 1 x 24 hours for 14 days. At the end of the study the rats were euthanized and the rats' kidneys were taken for the calculation of TNF-α expression using immunohistochemical staining and the number of inflammatory cells using hematoxylin-eosin staining. Analysis of quantitative data on TNF-α expression and the inflammatory cells count using One Way ANOVA test. The results showed that the extract of Blueberry could reduce TNF-α expression and the number of inflammatory cells in the kidney with the best dose of 1500 mg/kg. The conclusion is the blueberry extract has a therapeutic effect on acute kidney injury based on TNF-α expression and the number of inflammatory cells

Keywords: acute kidney injury, Blueberry, TNF-α, Inflammatory cell count