Escherichia coli O157: H7 is one of the foodborne pathogens that can be transmitted to humans. Calves (calf) infected by E. coli O157H7 show symptoms of mild diarrhea until bloody diarrhea. The antibiotics is a common therapy and has been chosen to treat diarrhea calf. However, it is now known that the E. coli strain has been resistant to many antibiotics. The purpose of this study was to determine the resistance profile of 15 types of antibiotics in 62 isolates of  E. coli O157H7 from cattle and one isolate of E. coli O157H7 ATCC 43984 as a control. Antibiotic sensitivity tests are carried out using agar diffusion techniques. Test results on 62 local isolates of E. coli O157H7 showed that 75.81% were resistant to Cephalotin, 69.35% were resistant to Sulphamethoxazole, 35.48% were resistant to Ampicillin, 25.81% were resistant to Amoxicillin/Clavulanic Acid, 14.52% resistant to Nalidixic Acid, 12.90% resistant to Streptomycin, 12.90% resistant to Kanamycin, 8.07% resistant to Tetracycline, 8.07% resistant to Trimethoprim, 4.84% resistant to Gentamycin, 4.84 % resistant to Ciprofloxacin, 3.23% isolates resistant to Norfloxacin, Enrofloxacin and Amoxicillin. While E. coli O157H7 ATCC 43984 isolate was resistant only to Sulphamethoxazole antibiotics, intermediates to Cephalotin and Streptomycin and were still sensitive to 12 antibiotics. The study also found 79.03% isolates resistant to 1-5 types of antibiotics, 4.84% resistant to 6-10 types of antibiotics and 4.84% resistant to 11-15 types of antibiotics. Based on the results of the study, it can be concluded that there are variations of multi-drug resistance in the local isolates of E. coli O157: H7 tested.

Key Words: resistance, antibiotic, Escherichia coli O157H7, cattle, diarrhea