ABSTRACT

Staphylococcus intermedius is a normal flora of dogs and also an opportunistic pathogen for common canine skin infections. The resistance of Staphylococcus intermedius to several antibiotics had been reported from several previous studies. Interaction of stray dogs and humans in Mandalika beach can mutually transmit Staphylococcus intermedius which is resistant to antibiotics.  This study aims to determine the bacterial resistance of Staphylococcus intermedius from several antibiotic groups. This Research is a laboratory exploration that has used two (2) Staphylococcus intermedius isolates that had been tested against three antibiotics (Tetracycline, Penicillin G, and Clindamycin). The resistance test was carried out by the BD Phoenix automated microbiology system. 1-4 colonies diluted with 0.5 ml of sterile physiological NaCl and dissolved into a phoenix ID Broth, Then measured by McFarland standard 0.5 µF based on the Clinical and Laboratory Standards Institute. Bacterial suspension put into the antimicrobial susceptibility testing (AST) Broth and entered to BD Phoenix automated microbiology system for 24 hours. The minimal inhibitory concentration (MIC) as antimicrobial susceptibility of antibiotic automated viewing on panel of BD Phoenix automated microbiology system. Interpretation result of MIC according to CSLI standard. The result showed that 2 Staphylococcus intermedius isolates were sensitive to tetracycline (100%) and  Clindamycin (100%) and only one of two isolates of Staphylococcus intermedius was resistant to groups beta-lactam as in Penicillin G (50%).

Keywords:, Antibiotic resistance, dog, Mandalika Dog Shelter, Staphylococcus intermedius