Abstract. Pork meat is one of livestock product that is the most important reservoir for Escherichia coli (E. coli). The incidence of antibiotics resistant E. coli that is isolated from pork has been widely reported around the world. The aim of our study was to investigate antibiotic resistant in E. coli isolated from pork meat sold in Tamiang Layang Traditional Market, East Barito Regency. A total of 41 samples from the upper of the ham were taken from Tamiang Layang Traditional Market. Isolation and identification of E. coli were carried out using Brilliance E. coli/Coliform selective Agar, Eosin Methylene Blue Agar (EMBA), and confirmed with kit Analytical Profile Index (API) 20E. The resistance against 10 antibiotics (nalidixic acid, erythromycin, ampicillin, streptomycin, penicillin G, sulfamethoxazole, ciprofloxacin, tetracycline, amoxicillin, and chloramphenicol) were tested using Kirby Bauer method, based on the standard of Clinical Laboratory Standards Institute (CLSI) in 2012. The result showed that 17 samples (41.5%) were positive containing E. coli and they were all resistant to erythromycin, streptomycin, penicillin G, and chloramphenicol (100%), tetracycline (94.1%), ampicillin (76.5%), and nalidixic acid (23.5%). All isolates were also resistant to more than 3 classes of antibiotics which were known as multi drug resistant (MDR) with mostly pattern of E-AMP-S-P-TE-C (erythromycin, ampicillin, streptomycin, penicillin G, tetracycline, and chloramphenicol).

Keywords: antibiotic resistant, E. coli, multidrug resistant, pork meat