Antibiotic resistance is emerging and spreading globally, threatening the ability to treat infectious diseases and becoming a global health threat.  This study was aimed to determine the occurrence of antibiotic resistance in Escherichia coli (E. coli) isolated from raw milk in Kebon Pedes, Bogor. The antibiotic resistance of E. coli in milk was done by evaluating 23 bulk raw milk samples from dairy farms in Kebon Pedes, Bogor. Isolation and identification of E. coli were referred to the SNI 2897:2008 about Guideline for Laboratory Analysis on Examination of Microbial Contamination in Meat, Egg, and Milk issued by the National Standardization Agency of Indonesia. The resistance against six antibiotics, i.e., amoxicillin, streptomycin, ciprofloxacin, tetracycline, and penicillin G, was tested using Kirby-Bauer disk diffusion method based on the standard of Clinical Laboratory Standards Institute (CLSI) in 2018. The result showed that 62 out of 207 (29.95%) isolates were found to be positive of E. coli. E. coli showed resistance to antibiotics, i.e., penicillin G (95.2%), streptomycin (84.3%), amoxicillin (74.2%), and tetracycline (62.5%).  77.42% of positive E. coli isolates were resistant to more than three classes of antibiotics, known as multi-drug resistant (MDR), with most patterns of AML-S-TE-P (amoxicillin, streptomycin, tetracycline, and penicillin G). The presence of E. coli resistant antibiotic in raw milk could result in serious problems in human health.

Keywords: antibiotic resistance, Escherichia coli, milk, multi-drug resistant